The Anti-PDL1 can help with programmed cell death and is also referred to as a cluster of differentiation or B7 homolog. It is a type 1 transmembrane protein used to regulate the humoral and cellular immune responses. The interaction of PDL-1 and PD-1 provides inhibitory and stimulatory signals for controlling T-cell tolerance and activation during pregnancy, autoimmune disease, malignant transformation, and tissue allografts. It is primarily expressed in activated T/B cells, antigen presenting cells, some tumors, and placenta.
It is a synthetic peptide that is derived from the C-terminus of the human PD-L1 protein. It uses a Rabbit IgG isotype and has an undetermined epitope. The molecular weight is 32 kDa, and it has a clone of SP142.
Applications
It is designed for use with Immunohistochemistry applications. The Anti-PDL1 uses the placenta as its positive control with a cellular localization in the membrane.
To prepare your specimen, it is best to use a Formalin-fixed, paraffin embedded tissue. Slides must be deparaffinized with xylene or an appropriate alternative, as well as graded alcohols.
When using the concentrated version of the product, you should dilute the antibody using a ratio of 1:100, though this is an estimation. Your studies and research may require different dilutions.
To retrieve the antigen, you should boil the tissue sections in an EDTA buffer with a pH of 8.0 for 10 full minutes, allowing it to cool to room temperature for at least 20 minutes. Afterward, you can start the incubation process for 10 minutes while at room temperature.
PBS-Tween can be used to rinse the slides after they’ve been washed between each step. Your visualization system and instructions will help you detect the antibody.
The Anti-PDL1 is available from Spring Bioscience for those researching the placenta and things during pregnancy. Visit them now for more information.
